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专利名称：Bacterial surface display and screening of
thioether-bridge-containing peptides
发明人：Tjibbe Bosma申请号：US13808206申请日：20110706公开号：US09651558B2公开日：20170516
摘要：The invention relates to bacterial cell surface display of post-translationallymodified heterologous proteins. Provided is an isolated nucleic acid construct encoding aproteinaceous substance comprising, from the N-terminus to the C-terminus, at least (a)an N-terminal a lantibiotic leader sequence; (b) an amino acid sequence of interest to bepost-translationally modified to a dehydroresidue- or thioether-bridge containingpolypeptide; (c) a hydrophilic cell-wall spanning domain; (d) a sortase recognition motif;(e) a hydrophobic membrane spanning domain and (f) a C-terminal charged membraneanchoring domain. Also provided is a Gram-positive host cell expressing the construct, aswell as a library of host cells.
申请人：Tjibbe Bosma
地址：Lippenhuizen NL
国籍：NL
代理机构：Licata & Tyrrell P.C.
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