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专利名称:DNA AMPLIFICATION VIA SCISSOR-LIKE
STRUCTURES (DASL)
发明人:LING, Mingfu申请号:EP14834501.0申请日:20140731公开号:EP3030669A1公开日:20160615
摘要:Methods and systems are provided for amplifying target nucleic acids by meansof a polymerase with strand displacement activity, using two or more stem loop primers,each with a 3′-end portion comprising sequence complementary to a target homologysite of a target nucleic acid and 5′-end portion with a sequence comprising a stemsequence, a loop sequence and a sequence which is reverse complementary to the stemsequence, the said 5′-end portion being capable of forming a stem loop. Such methodsand systems can be used to amplify target nucleic acids isothermally by means of apolymerase with strand displacement activities.
申请人:Genebio Systems Inc.
地址:80 Paulart Drive Toronto, Ontario M9B 3V9 CA
国籍:CA
代理机构:Errico, Michela
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